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Certification of the 17α-Trenbolone mass concentration in lyophilised bovine liver reference materials
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Certification of the 17α-Trenbolone mass concentration in lyophilised bovine liver reference materials

BCR-474 (Trenbolone free) and BCR-475 (Trenbolone positive)

Väljaande metaandmed

Trenbolone (176β-hydroxy-19-norandrosta-4,9,11-trien-3-one) acetate is an androgenic anabolizing agent used in meat production. Its use is permitted in a number of third countries including the United States of America but is banned in the European Union (Directive 96/22/EC). Trenbolone (TbOH) residues are controlled in urine, faeces, liver, fat and muscle samples. To harmonize the analytical performances within the EU, the « Standards , Measurements and Testing Programme » has funded the development of a series of Certified Reference Materials for veterinary drugs in animal tissues and fluids. This contribution describes the production and certification of lyophilised bovine liver materials uncontaminated (BCR-474, trenbolone-free, blank material) or contaminated with trenbolone (TbOH) residues (BCR-475 ; target value : 5.0 µg of α-TbOH/kg of fresh liver equivalent). Purified extracts were quantitatively analysed by two methods : RIA (radio-immunoassay) using an anti-α-TbOH antiserum (limit of detection = 0.7 µg/kg). GC-MS (gas chromatography-mass spectrometry) of MO-TMS (methyloxime-trimethylsilyl) derivatives (limit of detection = 0.5 µg/kg). A study to determine the homogeneity of TbOH residues in liver from treated and untreated animals indicated that the residues are normally distributed and thus that the materials are homogeneous. A stability study at various temperatures (-50, -18, +4, +20 and +37 °C ) indicated that the materials are stable. The results of the certification study demonstrated on one hand the fact that many laboratories still have significant problems with the analysis of samples of liver for Trenbolone. However, at the same time laboratories that do have a validated method operational showed to be able to produce good results. The certified values obtained for the CRMs are: BCR-474 (blank)< 0.5 µg/kg ; BCR-475 (positive) 7.6 ug/kg, uncertainty 2.2 ug/kg.
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ISBN 92-894-4384-7
Katalooginumber KI-NA-20-488-EN-C
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ISSN ISBN 92-894-4384-7 DOI Katalooginumber KI-NA-20-488-EN-C
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