Acquisition of a compact equipment for the analysis of molecular interactions and associated kinetic parameters, based on SPR for the University of the Balearic Islands, financed by the project EQC2024-008200-P, State Subprogramme for Research Infrastructures and Scientific-Technical Equipment within the framework of the State plan for scientific and technical research and innovation 2021-2023 Text automatically translated in your browsing language Automatically translated

The acquisition of a molecular interaction analyser using surface plasmon resonance or SPR (Surface Plasmon Resonance) is required. This type of technology allows the analysis of molecular interactions in real time without the need to use any type of marking (radioactivity, fluorescence, etc.). This type of technology does not exist at the University of the Balearic Islands (UIB). This RPS equipment will require a computer control, which implies that it is necessary to supply the computer equipment described in the TPP. SPR is a powerful analytical technique that can be applied to the study of different aspects in a variety of areas of knowledge. Its main application is the study of biomolecular interactions. Here are some of the main applications that UIB research groups would be interested in: 1.-Kinetic analysis of biomolecular interactions: SPR technology can be used to analyze the kinetics of interactions between biomolecules. Therefore, it can be used to study affinity, binding kinetics (constants of association and dissociation) and specificity between biomolecules such as proteins, peptides or nucleic acids and small molecules. 2.-Evaluation of possible drugs: Typically, SPR analysis has been widely used for the discovery and study of low molecular weight molecules with pharmacological potential. Analyzing the binding kinetics and the affinity of small molecules or compounds towards their protein receptors, it is possible to identify compounds that present the desired binding characteristics to a therapeutic target. This accelerates the process of drug discovery and development. 3.-Characterization of protein-protein, protein-lipid and protein-nucleic acid interactions: SPR technology has been widely used by the scientific community to quantify the affinity and specificity of this type of interaction, a fact that provides information on the molecular mechanisms that control biological processes. Within protein-protein interactions, the antigen-antibody interaction should be taken into account, which is studied, not only in basic and applied immunological research, but also in the development and optimization of vaccines. Finally, protein-nucleic acid interaction is studied in genetic research and development of gene therapies. 4.- Research of molecular interactions of interest in biotechnology: In addition to the interest generated by research in the interaction of biomolecules or the characterisation of interactions of pharmacological interest, interactions of interest in the biotechnological field, such as the interaction of specific proteins or enzymes with plastic polymers (polypropylene, polyethylene and polystyrene) and with other synthetic derivatives present in the environment such as pesticides, antibiotics or heavy metals, which may be found in the environment and in consumer products, should also be considered. 5.-Mass testeo: The SPR technology, thanks to its short work cycles, allows to test many molecules at different concentrations on the same receptor in acceptable periods of time, thus obtaining a high experimental performance of each test with a certain immobilized receptor. This application can take place in many areas of knowledge. Text automatically translated in your browsing language Automatically translated

38434500 - Biochemical analysers