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Today17/01/2025
Utilities
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Voluntary ex ante transparency: nLC-MSMS system Text automatically translated in your browsing language Automatically translated
Wageningen University needs a contract for the supply of a high-resolution mass spectrometer with nano flow liquid chromatography. We ask for an overview of interested and experienced market parties who can supply a system with the specifications as described under section 11 for a maximum amount of € 800,000 including VAT. The goal of an additional mass spectrometry system for the facility is to increase the current measurement capacity due to greater demand for qualitative and quantitative proteomics data. In order to meet these requirements, we set the following requirements for the system: For accurate peptide and post translational modification identification and quantification a minimum resolution of 240000 at a m/z of 200. The mass deviation must be on average less than 1 ppm for a minimum of 5 to a closed day for the purpose of measuring larger (high throughput) data sets. The mass range shall be between 40 and 6000 m/z and the MS/MS measurement speed shall be at least 20 Hz. For accurate quantification, the system shall have sufficient resolution to measure 18 plex TMT or more. Various scan modes must be available such as data dependent acquisition (DDA), data independent acquisition (DIA) and parallel response monitoring (PRM). Due to lack of space capacity, the system must have a small footprint or bench top model. The nanoflow liquid chromatography shall meet the following requirements: Have a flow control from 1nl/min to 100μl/min. In order to maximize the measuring capacity and optimal use of the MSMS capacity, the system must be able to facilitate two columns. It must be possible to connect custom-made columns and adjust the liquid gradients themselves and flexibly. Furthermore, the system has connections and adjustments that minimize dead volume (e.g. Viper and nanoViper connections). Text automatically translated in your browsing language Automatically translated
38000000 - Laboratory, optical and precision equipments (excl. glasses)
38433000 - Spectrometers CVP code deduced from the text of the procedure AI-generated
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