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HomeCertification of a reference material with Candida albicans (NCPF 3179) at a target level of 1000 colony forming units per material sphere IRMM-354
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This report describes the certification of a reference material (IRMM-354) of Candida albicans. Certified Reference Materials (CRMs) for microbiological analysis are indispensable tools for development and validation of detection methods and for the implementation and support of internal and external quality control in the area of microbiological analysis. Each vial of IRMM-354 contains one material sphere of Candida albicans. The homogeneity and stability (at -20 °C and -70 °C) of the batch was assessed by counting colony forming units (cfu) per material sphere on nutrient agar (NA) and Oxytetracyclin-Glucose-Yeast Extract (OGYE) agar. The material is stable when stored at a maximum temperature of -20 °C for up to 18 months. The batch was characterised by six laboratories to establish a certified value of cfu per material sphere on NA and OGYE agar. The certified value for NA is 917 cfu per material sphere with an expanded uncertainty of 168. The certified value for OGYE agar is 912 cfu per material sphere with an expanded uncertainty of 173. In both cases, a coverage factor k = 2 is used, corresponding to a level of confidence of about 95 %. The authenticity of the material was confirmed by DNA sequence analysis of the coding region for the hwp1 gene.
- Published:
- Corporate author(s): Institute for Reference Materials and Measurements (Joint Research Centre)
- Personal author(s): De Baets, L ; Meeus, N ; Schimmel, H
- Themes: Medical and biological research
- Subject: biology , Community certification , research body , research method
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| ISSN 1018-5593 | ISBN 978-92-79-11254-6 | DOI 10.2787/19017 | Catalogue number LA-NA-23707-EN-C |
- Released on EU publications website:
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