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HomeCertification of C-reactive protein in reference material ERM-DA472/IFCC certified reference materials ERM-DA472/IFCC
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The production and certification of ERM-DA472/IFCC, a new reference material certified for C-reactive protein (CRP), is described. Serum was produced from blood collected in 6 blood collection centres according to a procedure ensuring that it was obtained from healthy donors, and that the lipid content of the serum was low. The serum was processed in 5 batches, and then pooled, spiked with β2 microglobulin (B2M) and CRP and filled into ampoules (approximately 1 mL serum per ampoule). The serum was stored at -70 °C. It was verified that the material was homogenous and stable. ERM-DA472/IFCC was characterised using the reference material ERM-DA470 as calibrant. This was achieved using a value transfer protocol that can be considered as a reference procedure. The principles used to measure the CRP concentration were immunonephelometry and immunoturbidimetry. The measurements were performed with different platform/reagent combinations (Abbott Architect, Beckman Immage, BN II, different Hitachi instruments, and Olympus AU640). In total 8 laboratories participated in the value assignment. The certified CRP mass concentration is 41.8 mg/L, the expanded uncertainty (k = 2) 2.5 mg/L.
- Published:
- Corporate author(s): Institute for Reference Materials and Measurements (Joint Research Centre)
- Personal author(s): Charoud-Got, J ; Zegers, I ; Rzychon, M
- Themes: Medical and biological research
- Subject: analytical chemistry , Community certification , medical research , research body , research method
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| ISSN 1018-5593 | ISBN 978-92-79-11326-0 | DOI 10.2787/21201 | Catalogue number LA-NA-23756-EN-C |
- Released on EU publications website:
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