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HomeReport on the single-laboratory validation of a PCR-based detection method for identification of Florigene 26407 GM carnation
Метаданни за публикацията
Suntory Holdings Ltd has submitted an application for marketing (C/NL/09/02) of a genetically modified carnation line 26407 (Unique identifier: IFD-26407-2). In this context, the European Union Reference Laboratory for GM Food and Feed (EU RL GMFF) was asked to carry out a single-laboratory validation of the performance of a polymerase chain reaction (PCR)-based method for detecting and identifying the carnation GM line 26407, developed by the applicant. This report describes the results of this validation, carried out by the EU RL GMFF with control samples provided by the applicant. The method is a duplex end-point PCR, where a carnation (taxon) target and a transgenic sequence are detected simultaneously. The limit of detection (LOD) of the method has been established to be at least 10 copies for the taxon-specific target and between 50 and 10 copies for the GM target, based on haploid genome copy number. The event-specificity of the method was assessed by the applicant as being sufficient. The EU-RL could verify that the taxon-specific primers correctly detect the endogenous gene target in genomic DNA of a conventional carnation line (negative control) and in the genomic DNA of the GM carnation line, while the GM target is detected by the GM specific primers only in genomic DNA of 26407 GM line (positive control).
- Публикувано:
- Корпоративен aвтор: Institute for Health and Consumer Protection (Съвместен изследователски център)
- Теми: Технология на храните и безопасност на храните
- Тема: биотехнология , генетика , генно модифицирано растение , доклад от научно изследване , изследователски метод , хранене
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| ISSN 1831-9424 | ISBN 978-92-79-34531-9 | DOI 10.2788/34771 | Каталожен номер LB-NA-26290-EN-N |
- Released on EU publications website:
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